The favorite to take the gold medal in London has suffered a tear to his Achilles just six months before the Olympic Games.
The 23-year-old from Yorkshire must now wear a protective boot for a month.
“If I can get this boot off quite quickly and get training, I can get myself fit in time [for the Olympics],” Brownlee told the Metro newspaper.
Brownlee is expected to compete next at the San Diego World Triathlon Series event in May.
Malcolm Brown, one of Brownlee’s personal coaches and British Triathlon’s Olympic performance group manager, said: “Alistair has a small tear in his left Achilles tendon and is wearing an air cast as a precaution.
“Obviously this isn’t an ideal situation, but I can say that this has been his best winter’s training, probably ever. We had scheduled a break from training at the end of the winter period anyway, so I am not overly concerned at this stage.”
Read more: Bbc.co.uk